Dye Fading Kinetics updated 3/1/2005

1. It is possible to trap a bubble under the sensor. Try not to do this, because it makes the readings go crazy.
2. You need a final equilibrium concentration in order to analyze the data. Make sure that you reach this equilibrium concentration for enough time to determine a value.
3. The initial readings versus time always bounce around a little bit. You should be able to explain why. This necessitates extrapolating back to an initial value.
4. You may assume first-order reversible kinetics.
5. Set the time that the spectrophotometer collects data for a lot longer than you think. You can always stop it early. Previous students have found that at least 90 minutes are required for the lowest temperature settings. Shorter times are required for higher temperatures (this should be obvious).